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syrian hamster fibroblast cell line bhk21 cells  (ATCC)


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    ATCC syrian hamster fibroblast cell line bhk21 cells
    Syrian Hamster Fibroblast Cell Line Bhk21 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hamster+fibroblast+cell+line+bhk21/BHK-21/pm39232285-71-9-6
    Average 99 stars, based on 5182 article reviews
    syrian hamster fibroblast cell line bhk21 cells - by Bioz Stars, 2026-09
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    Transformation Assay:

    Article Title: Structural insights into the modulation of coronavirus spike tilting and infectivity by hinge glycans.
    Article Snippet: 4–15% Mini-PROTEAN® TGXTM Precast Protein Gels, 15-well, 15 μl (BioRad); SimplyBlueTM SafeStain (Invitrogen); dithiothreitol (Sigma); iodoacetamide (Sigma); alpha lytic protease (New England BioLabs); chymotrypsin (Athens Research and Technology); AspN (Promega); Glu-C (Promega); trypsin (Promega); endoglycosidase H (Promega); PNGaseF (Promega); 18O water (Cambridge Isotope Laboratories). .. MA104 (ATCC CRL-2378.1) cells were maintained at 37 °C in a fully humidified atmosphere with 5% CO2 in M199 (Gibco) medium, hamster fibroblast cell line BHK21 (ATCC CCL-10) and adenovirus 5 DNA transformed human kidney epithelial cell line 293 c18 (ATCC CRL-10852) were maintained at 37 °C in a fully humidified atmosphere with 5% CO2 in DMEM (Gibco). .. All culture media were supplemented with penicillin and streptomycin and 10% FBS (Hyclone).

    Article Title: Structural insights into the modulation of coronavirus spike tilting and infectivity by hinge glycans
    Article Snippet: 4–15% Mini-PROTEAN® TGXTM Precast Protein Gels, 15-well, 15 μl (Bio-Rad); SimplyBlueTM SafeStain (Invitrogen); dithiothreitol (Sigma); iodoacetamide (Sigma); alpha lytic protease (New England BioLabs); chymotrypsin (Athens Research and Technology); AspN (Promega); Glu-C (Promega); trypsin (Promega); endoglycosidase H (Promega); PNGaseF (Promega); 18O water (Cambridge Isotope Laboratories). .. MA104 (ATCC CRL-2378.1) cells were maintained at 37 °C in a fully humidified atmosphere with 5% CO 2 in M199 (Gibco) medium, hamster fibroblast cell line BHK21 (ATCC CCL-10) and adenovirus 5 DNA transformed human kidney epithelial cell line 293 c18 (ATCC CRL-10852) were maintained at 37 °C in a fully humidified atmosphere with 5% CO 2 in DMEM (Gibco). .. All culture media were supplemented with penicillin and streptomycin and 10% FBS (Hyclone).



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    syrian hamster fibroblast cell line bhk21 cells  (ATCC)
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    hamster fibroblast cell line bhk21  (ATCC)
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    baby hamster kidney fibroblast cell line bhk21  (ATCC)
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    Initial design of SARS-CoV-2 replicons. (A) Top, genome organization of SARS-CoV-2. Leader sequence (red), transcriptional regulatory sequence within the leader sequence (TRS-L) and within the body (TRS-B) are highlighted in green. Bottom, the design of SARS-CoV-2-Rep-NanoLuc-Neo. (B, C, D, E) Replication kinetics of SARS-CoV-2-Rep-NanoLuc-Neo in Vero E6 (B), A549 (C), Huh7.5.1 (D), and BHK-21 cells (E). Nano luciferase was measured at indicated time points post-electroporation. Notably, nanoluciferase continued to decrease even under G418 selection. (F) Generation of <t>BHK21</t> stable cells that express NP in a doxycycline-inducible manner. Cells were induced with 0.5 μg/mL doxycycline and lysed at 48 h postinduction for Western blotting with anti-NP and anti-actin antibodies. Numbers on the left refer to the positions of marker proteins that are given in kilodalton (kDa).
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    Initial design of SARS-CoV-2 replicons. (A) Top, genome organization of SARS-CoV-2. Leader sequence (red), transcriptional regulatory sequence within the leader sequence (TRS-L) and within the body (TRS-B) are highlighted in green. Bottom, the design of SARS-CoV-2-Rep-NanoLuc-Neo. (B, C, D, E) Replication kinetics of SARS-CoV-2-Rep-NanoLuc-Neo in Vero E6 (B), A549 (C), Huh7.5.1 (D), and BHK-21 cells (E). Nano luciferase was measured at indicated time points post-electroporation. Notably, nanoluciferase continued to decrease even under G418 selection. (F) Generation of BHK21 stable cells that express NP in a doxycycline-inducible manner. Cells were induced with 0.5 μg/mL doxycycline and lysed at 48 h postinduction for Western blotting with anti-NP and anti-actin antibodies. Numbers on the left refer to the positions of marker proteins that are given in kilodalton (kDa).

    Journal: Journal of Virology

    Article Title: Stable Cell Clones Harboring Self-Replicating SARS-CoV-2 RNAs for Drug Screen

    doi: 10.1128/jvi.02216-21

    Figure Lengend Snippet: Initial design of SARS-CoV-2 replicons. (A) Top, genome organization of SARS-CoV-2. Leader sequence (red), transcriptional regulatory sequence within the leader sequence (TRS-L) and within the body (TRS-B) are highlighted in green. Bottom, the design of SARS-CoV-2-Rep-NanoLuc-Neo. (B, C, D, E) Replication kinetics of SARS-CoV-2-Rep-NanoLuc-Neo in Vero E6 (B), A549 (C), Huh7.5.1 (D), and BHK-21 cells (E). Nano luciferase was measured at indicated time points post-electroporation. Notably, nanoluciferase continued to decrease even under G418 selection. (F) Generation of BHK21 stable cells that express NP in a doxycycline-inducible manner. Cells were induced with 0.5 μg/mL doxycycline and lysed at 48 h postinduction for Western blotting with anti-NP and anti-actin antibodies. Numbers on the left refer to the positions of marker proteins that are given in kilodalton (kDa).

    Article Snippet: The baby hamster kidney fibroblast cell line BHK21 (CCL-10), African green monkey kidney epithelial cells (Vero E6; CRL-1586), Caco-2 (HTB-37), Calu-3 (HTB-55), and A549 (CCL-185) were purchased from the American Type Culture Collection.

    Techniques: Sequencing, Luciferase, Electroporation, Selection, Western Blot, Marker

    Optimization of SARS-CoV-2 replicons. (A) Illustration of Nsp1 binding to the small ribosomal subunit (PDB code:7K5I). Nsp1 (orange) binds close to the mRNA entry site and contacts uS3 (green) from the ribosomal 40S head as well as uS5 (blue), and h18 of the 18S rRNA (charcoal gray) of the 40S body. The fragment of rRNA not close to Nsp1 is shown transparently. (B) An enlarged view of the Nsp1 binding area. Critical residues are shown in the stick representation and are labeled. (C) Calculated free energy changes (ΔΔG) for various mutations in Nsp1. Positive values indicate unfavorable mutations for the binding between Nsp1 and rRNA. (D) Three pairs of Nsp1 mutations were introduced to SARS-CoV-2-Rep-NanoLuc-Neo to obtain three new replicons. Figures are not drawn in proportion. (E) BHK21-NP Dox-ON cells were transiently transfected with Rep-NanoLuc-Neo-Nsp1 R124S/K125E RNA, Rep-NanoLuc-Neo-Nsp1 N128S/K129E RNA and Rep-NanoLuc-Neo-Nsp1 K164A/H165A RNA. Nano luciferase was measured at indicated time points posttransfection in the absence of G418. (F) Replication kinetics of Rep-NanoLuc-Neo-Nsp1 K164A/H165A in Huh7.5.1 cells. Electroporated cells were lysed at indicated time points (without G418) posttransfection for nanoluciferase quantification.

    Journal: Journal of Virology

    Article Title: Stable Cell Clones Harboring Self-Replicating SARS-CoV-2 RNAs for Drug Screen

    doi: 10.1128/jvi.02216-21

    Figure Lengend Snippet: Optimization of SARS-CoV-2 replicons. (A) Illustration of Nsp1 binding to the small ribosomal subunit (PDB code:7K5I). Nsp1 (orange) binds close to the mRNA entry site and contacts uS3 (green) from the ribosomal 40S head as well as uS5 (blue), and h18 of the 18S rRNA (charcoal gray) of the 40S body. The fragment of rRNA not close to Nsp1 is shown transparently. (B) An enlarged view of the Nsp1 binding area. Critical residues are shown in the stick representation and are labeled. (C) Calculated free energy changes (ΔΔG) for various mutations in Nsp1. Positive values indicate unfavorable mutations for the binding between Nsp1 and rRNA. (D) Three pairs of Nsp1 mutations were introduced to SARS-CoV-2-Rep-NanoLuc-Neo to obtain three new replicons. Figures are not drawn in proportion. (E) BHK21-NP Dox-ON cells were transiently transfected with Rep-NanoLuc-Neo-Nsp1 R124S/K125E RNA, Rep-NanoLuc-Neo-Nsp1 N128S/K129E RNA and Rep-NanoLuc-Neo-Nsp1 K164A/H165A RNA. Nano luciferase was measured at indicated time points posttransfection in the absence of G418. (F) Replication kinetics of Rep-NanoLuc-Neo-Nsp1 K164A/H165A in Huh7.5.1 cells. Electroporated cells were lysed at indicated time points (without G418) posttransfection for nanoluciferase quantification.

    Article Snippet: The baby hamster kidney fibroblast cell line BHK21 (CCL-10), African green monkey kidney epithelial cells (Vero E6; CRL-1586), Caco-2 (HTB-37), Calu-3 (HTB-55), and A549 (CCL-185) were purchased from the American Type Culture Collection.

    Techniques: Binding Assay, Labeling, Transfection, Luciferase

    Characterization of cells harboring BHK21-NPDox-ON Rep-NanoLuc-Neo-Nsp1 K164A/H165A . (A) Nano luciferase in BHK21-NP Dox-ON replicon cells (Pool #1) was measured at given time points following G418 withdrawal. RNA was also extracted at indicate time points and quantified by RT-qPCRs targeting ORF1ab (gRNA in A), or sgmNeoR and sgmNanoLuc (B). (C) RT-PCR analysis of viral RNA from replicon Pool #1 cells. The corresponding primer pairs are shown in the right table. The lengths of DNA fragments are indicated in the table to the right. Fragments detected in Lanes 1 to 6 are from different regions of ORF1ab; fragments in lane 7 and 8 represent subgenomic RNAs of the NanoLuc and E (NeoR in replicon). (D) Western blot analysis of the SARS-CoV-2 proteins from Pool #1 and six representative stable replicon clones. The presence of NP and Nsp1 protein in cell lysates was confirmed. BHK-21 and BHK21-NP Dox-ON (without doxycycline induction) were included as negative controls. (E) Sequence coverage of canonical sgmRNA species in Pool #1 and Pool #2 replicon cells as well as in 12 stable clones.

    Journal: Journal of Virology

    Article Title: Stable Cell Clones Harboring Self-Replicating SARS-CoV-2 RNAs for Drug Screen

    doi: 10.1128/jvi.02216-21

    Figure Lengend Snippet: Characterization of cells harboring BHK21-NPDox-ON Rep-NanoLuc-Neo-Nsp1 K164A/H165A . (A) Nano luciferase in BHK21-NP Dox-ON replicon cells (Pool #1) was measured at given time points following G418 withdrawal. RNA was also extracted at indicate time points and quantified by RT-qPCRs targeting ORF1ab (gRNA in A), or sgmNeoR and sgmNanoLuc (B). (C) RT-PCR analysis of viral RNA from replicon Pool #1 cells. The corresponding primer pairs are shown in the right table. The lengths of DNA fragments are indicated in the table to the right. Fragments detected in Lanes 1 to 6 are from different regions of ORF1ab; fragments in lane 7 and 8 represent subgenomic RNAs of the NanoLuc and E (NeoR in replicon). (D) Western blot analysis of the SARS-CoV-2 proteins from Pool #1 and six representative stable replicon clones. The presence of NP and Nsp1 protein in cell lysates was confirmed. BHK-21 and BHK21-NP Dox-ON (without doxycycline induction) were included as negative controls. (E) Sequence coverage of canonical sgmRNA species in Pool #1 and Pool #2 replicon cells as well as in 12 stable clones.

    Article Snippet: The baby hamster kidney fibroblast cell line BHK21 (CCL-10), African green monkey kidney epithelial cells (Vero E6; CRL-1586), Caco-2 (HTB-37), Calu-3 (HTB-55), and A549 (CCL-185) were purchased from the American Type Culture Collection.

    Techniques: Luciferase, Reverse Transcription Polymerase Chain Reaction, Western Blot, Clone Assay, Sequencing